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Sialic Acid Analysis

Sialic Acids are most often found at the terminal position of glycans, and therefore are a likely point of contact for glycan-ligand interactions.

Biopharmaceutical sialylation refers to the type and distribution of sialic acids in the glycans of therapeutic glycoproteins. Sialylation can significantly influence the safety and efficacy profiles of these drugs. In particular, the in vivo half-life of some biopharmaceuticals correlates with the degree of oligosaccharide sialylation.

Furthermore, the sialylation pattern can be a very useful measure of product consistency during manufacturing. Advances in our understanding of these issues have led the FDA, EMEA and other regulators to tighten their rules on glycoprofiling throughout the drug life cycle.

The two main types of sialyl residues found in biopharmaceuticals produced in mammalian expression systems are N-acetyl-neuraminic acid (NeuAc) and N-glycolylneuraminic acid (NeuGc). These usually occur as terminal structures attached to galactose (Gal) residues at the non-reducing terminii of both N- and O-linked glycans.

Controlling the ratio of NeuAc to NeuGc is critical for biomanufacturers. The reason is that NeuAc is the desired, normal human-type sialylation, while NeuGc is found in non-human glycoproteins and is considered an undesired, aberrant form of sialylation for therapeutic glycoproteins.

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DMB Sialic Acid Labeling Kit

The LudgerTag DMB Labeling Kit has been developed for the qualitative analysis of sialic acids. The kit includes all the reagents to release sialic acids from your glycoprotein and label these with DMB. View product documentation as PDF View Certificate of Analysis as PDF Part Number: LT-KDMB-A1

uR2 2.1x100mm UHPLC Column

Developed for the analysis of sialic acids labeled with 1,2-diamino-4,5 methylenedioxybenzene.2HCl (DMB) using UHPLC.

Dimensions: 2.1mm x 100mm

View product documentation as PDF

View Certificate of Analysis as PDF

Part Number: LS-UR2-2.1x100

$1,577.00

Sialic Acid Quantitation Kit

The SialiQuant Sialic Acid Quantitation Kit contains all the reagents to quickly and accurately quantitate sialic acids, including N-acetylneuraminic acid (NANA), N-glycolylneuraminic acid (NGNA).

View product documentation as PDF

Part Number: KE-SIALIQ

Sialic Acid Aldolase

Converts sialic acid to N-acetylmannosamine & pyruvate. Sialic acids can then be quantitated by a coupled reaction with NADH and lactic dehydrogenase

View product documentation as PDF

Part Number: E-ALD01

CMP-Sialic Acid Synthetase

This enzyme catalyzes the synthesis of CMP sialic acid from CTP & sialic acid.
The resulting molecule can then be covalently attached to an oligosaccharide by sialic acid transferase.

View product documentation as PDF

Part Number: E-CMP01

Sialidase Au
Alpha-(2-3,6,8,9)

recombinant from A. ureafaciens

View product documentation as PDF

Part Number: E-S001

Sialidase Cp
Alpha-(2-3,6)

recombinant from C. perfringens

View product documentation as PDF

Part Number: E-S005

Sialidase Sp
Alpha-(2-3)

recombinant from Streptococcus pneumoniae in E. Coli

View product documentation as PDF

Part Number: E-S007

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