This clean-up plate is used to enrich N-glycans prior to performing permethylation of released glycans. The 96 well plate is used for removal of excess salts, detergent, proteins and other impurities after the release of N-glycans from glycoproteins.
Part Number: LC-PERMET-96
The LC-PERMET-96 plate is a 96 well membrane-bottom plate containing a specialized glycan binding membrane. This product is designed for use with both the vacuum manifold that can be purchased from Ludger or with other popular vacuum manifold systems. Impurities from enzymatic N-glycan release can be washed through the membrane whilst glycans are bound to the membrane. The enriched glycans can be eluted in the end and dried down before permethylation.
Number of Samples Sufficient for up to 96 samples.
Volume of Samples Up to 350 µL per well.
Suitable Samples Any unlabelled glycans or glycans released from glycoproteins by PNGase F.
Storage Store at room temperature. Protect from sources of heat, light, and moisture. When stored correctly, the plate should be stable for 36 months from date of purchase.
Shipping The product should be shipped at ambient temperature.