IgG glycan reference Panel, monoclonal antibody reference panel
Description: A mixture of glycans commonly found on monoclonal antibodies includes G2F(NA2F), G0F(NGA2F), and both G1F isomers
Sources: The panel is a mixture of previously isolated glycans from various sources, as listed in the description of the individual glycans.
Form: Dry. Dried by centrifugal evaporation from an aqueous solution.
Molecular Weight: G2F:1787, G1F:1624, G0F:1463
Purity: > 90% pure as assessed by a combination of 1 H-NMR and HPLC, and CE
Storage: -20˚C both before and after dissolution. This product is stable for at least 5 years as supplied.
Shipping: The product can be shipped at ambient when dry. After dissolution, ship on dry ice.
Handling: Allow the unopened vial to reach ambient temperature and tap unopened on a solid surface to ensure that most of the lyophilized material is at the bottom of the vial. Gently remove the cap, add the desired volume of reconstitution medium, re-cap and mix thoroughly to bring all the oligosaccharide into solution. For maximal recovery of oligosaccharide, ensure that the cap lining is also rinsed and centrifuge the reconstituted vial briefly before use. Ensure that any glass, plasticware or solvents used are free of glycosidases and environmental carbohydrates. Minimise exposure to elevated temperatures or extremes of pH. High temperatures and low pH will cause desialylation. High pH will cause epimerisation of the reducing terminus GlcNAc.
Safety: This product is non-hazardous and has been purified from natural sources certified to be free of all hazardous material including pathogenic biological agents.