LudgerSep N Buffer
For preparation of LS-N Buffer (50 mM ammonium formate buffer, pH 4.4) used in amide or HILIC (hydrophilic interaction liquid chromatography) HPLC analysis of LudgerTag fluorophore and UV-chromophore labeled glycans.
Part Number: LS-N-BUFFX40
Use the LS-N buffer for glycan analysis with any of the following HPLC columns:
- LudgerSep N series columns
- Tosoh Amide-80 columns
- Waters ACQUITY UPLC BEH glycan columns
- GlycoSep N and N-Plus columns
- Other HILIC HPLC columns for glycan analysis
Includes 50ml of x40 LS-N buffer (2.0M ammonium formate buffer/solution) in a square HDPE bottle with leak-proof cap.
Dilute with de-gassed HPLC grade water (use 1 part of x40 buffer to 39 parts of water) to make LS-N buffer (50 mM ammonium formate, pH 4.4). The 50 ml of x40 buffer will make 2 litres of LS-N buffer.