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Endo F2

Selective release of biantennary and high mannose (at a 40X reduced rate) N-glycans from peptides and proteins

View product documentation as PDF

Part Number: E-EF02

$433.00

Part number E-EF02

Endo F2, Endoglycosidase F2, endo-beta-N-acetylglucosaminidase F2

Source recombinant Elizabethkingia miricola (was Chryseobacterium meningosepticum)in E. Coli

EC 3.2.1.96

Specifictity
Cleaves all asparagine-linked biantennary oligosaccharides and high mannose (though at a 40X reduced rate) N-glycans from peptides and proteins

Contents
60 µl aliquot of enzyme (0.3 U) in 10 mM sodium acetate 25mM NaCl, pH 4.5
5x Reaction Buffer – 250 mM sodium acetate, pH 4.5

Specific Activity >20 U/mg

Activity 5 U/ml

Molecular weight 32,000 daltons

Suggested usage
1. Add up to 200 µg of glycoprotein to an Eppendorf tube. Adjust to 38 µl final volume with de-ionized water.
2. Add 10 µl 5x Reaction Buffer 4.5
3. Add 2.0 µl of Endo F2. Incubate 1 hour at 37˚C.

Specific Activity
Defined as the amount of enzyme required to catalyze the release of N-linked oligosaccharides from 1 micromole of denatured Ribonuclease B (RNase B) in 1 minute at 37˚C, pH 5.5. Cleavage is monitored by SDS-PAGE (cleaved RNase B migrates faster).

Storage Store enzyme at 4˚C.

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