CMP-Sialic Acid Synthetase
This enzyme catalyzes the synthesis of CMP sialic acid from CTP & sialic acid.
The resulting molecule can then be covalently attached to an oligosaccharide by sialic acid transferase.
Part Number: E-CMP01
Source rec. from E.Coli
60 µl aliquot of enzyme ( 540 mU) as ammonium sulfate precipitate in 1 mM sodium phosphate, pH 7.5
1 vial reaction buffer- 200mM Tris-HCl, pH 9.5, 100 mM magnesium chloride
Specific Activity >2.5 U/mg
Activity 9 U/ml
Molecular weight ~46,000 daltons
Specificity:This enzyme catalyzes the synthesis of CMP sialic acid from CTP & sialic acid.
Specific Activity One unit of CMP-sialic acid synthetase is defined as the amount of enzyme required to produce 1 µmole of CMP-sialic acid in 1 minute at 37 ¡C, pH 9.5, from CTP & sialic acid.