Part number E-BG07
Source recombinant Streptococcus pneumoniae
60 µl aliquot of enzyme (180 mU) in 20 mM Tris-HCl, 25 mM NaCl (pH 7.5).
200 µl 5x Reaction Buffer 6.0 (250 mM sodium phosphate, pH 6.0).
Specific Activity >6 U/mg
Activity 3 U/ml
Molecular weight ~250,000 daltons
Suggested Usage Add 2 µL of enzyme to 100 µg of asialoglycoprotein or 1 nmol of oligosaccharide. Add buffer and incubate 1 hour at 37˚C.
Specifictity Non-reducing terminal Beta-(1-4)-galactose. Number of antennae does not affect cleavage rate. Fucose linked to the penultimate N-acetylglucosamine will block cleavage of the galactose.
Specific Activity One unit of Beta-(1-4)-galactosidase is defined as the amount of enzyme required to produce 1 µmole of p-nitrophenol (pNP) in 1 minute at 37˚C pH 5 from p-nitrophenyl-beta-D-galactopyranoside.
Storage Store enzyme at 4˚C.