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recombinant from Streptococcus pneumoniae

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Part Number: E-BG07


Part number E-BG07
Source recombinant Streptococcus pneumoniae

60 µl aliquot of enzyme (180 mU) in 20 mM Tris-HCl, 25 mM NaCl (pH 7.5).
200 µl 5x Reaction Buffer 6.0 (250 mM sodium phosphate, pH 6.0).

Specific Activity >6 U/mg
Activity 3 U/ml

Molecular weight ~250,000 daltons

Suggested Usage Add 2 µL of enzyme to 100 µg of asialoglycoprotein or 1 nmol of oligosaccharide. Add buffer and incubate 1 hour at 37˚C.

Specifictity Non-reducing terminal Beta-(1-4)-galactose. Number of antennae does not affect cleavage rate. Fucose linked to the penultimate N-acetylglucosamine will block cleavage of the galactose.

Specific Activity One unit of Beta-(1-4)-galactosidase is defined as the amount of enzyme required to produce 1 µmole of p-nitrophenol (pNP) in 1 minute at 37˚C pH 5 from p-nitrophenyl-beta-D-galactopyranoside.

Storage Store enzyme at 4˚C.

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