Part number E-BG02
Source Bovine Testes
200 µl aliquot of enzyme (0.5 U) in 20 mM Tris-HCl, 50 mM NaCl, 0.5mg/ml BSA,pH 7.5
5x Reaction Buffer- 500 mM sodium citrate/phosphate pH 4
Specific Activity >10.2 U/mg
Activity 2.5 U/ml
Molecular weight 68,000 daltons
Rxn pH 4
Add 2 µL of enzyme to 100 µg of asialoglycoprotein or 1 nmol of oligosaccharide. Add buffer and incubate 1 hour at 37˚C.
Specifictity Cleaves all Beta1-3 and Beta1-4 linked non-reducing, terminal galactose
Specific Activity One unit of Beta-(1-3,4,6)-Galactosidase is defined as the amount of enzyme required to produce 1 µmole of p-nitrophenol (pNP) in 1 minute at 37˚C, pH 4.0 from p-nitrophenyl-Beta-D-galactopyranoside.
Storage Store enzyme at -20˚C.