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2-AB Labeling Kit

2-AB (2-aminobenzamide) is one of the most widely used fluorescent labels for glycosylation analysis

Photo Guide of the Procedure

View product documentation as PDF

View Certificate of Analysis as PDF

Part Number: LT-KAB-A2

$539.00

Please note that there are a number of different conditions described in the literature for glycan labelling with 2-AB. This kit uses the conditions used by Bigge et al (1995). Please contact us if you would like to use other labeling conditions.

 

Kit Specifications


The kit contains two sets of the following reagents (15 samples per kit) supplied in glass ampoules sealed under pure nitrogen:

  • 2-aminobenzamide (2-AB dye)
  • Dimethyl sulfoxide (DMSO)
  • Acetic acid
  • Sodium cyanoborohydride

One kit contains reagents to label up to 30 separate analytical samples per kit.

Dye purity > 99% by HPLC
Molecular weight 137
lambda-ex = 320 nm
lambda-em = 420 nm

 

References


    1. Anumula KR, Dhume ST. (1998)
      High resolution and high sensitivity methods for oligosaccharide mapping and characterization by normal phase high performance liquid chromatography following derivatization with highly fluorescent anthranilic acid.
      Glycobiology 8: 685-694

 

    1. Bigge JC, Patel TP, Bruce JA, Goulding PN, Charles SM, Parekh RB. (1995)
      Nonselective and efficient fluorescent labeling of glycans using 2- amino benzamide and anthranilic acid.
      Anal Biochem 230: 229-238

 

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