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Glycan Labeling

LudgerTag kit reagents are purified to analytical grade and are dispensed and sealed under clean, inert atmospheres. The ampoules are pre-cleaned by pyrolysis at 500°C then opened just before dispensing and sealing under oxygen-free dry nitrogen. These controls ensure that your analyses work properly each and every time.

Acetic Acid – Promotes acid catalyzed ring opening of the glycan reducing terminus prior to the formation of an imine intermediate between the sugars and the unprotonated form of the dye

Fluorescent Dye – The comprehensive range of LudgerTag dyes include 2-AB, 2-AA, Procainamide, DMB, and APTS to cover different types of glycoanalyses by HPLC, CE, and MS.

DMSO – An anhdrous solvent used to dissolve all the components of the reductive amination labeling reaction.

Sodium Cyanoborohydride or Non-Toxic 2PB – This reductant stabilizes the imine produced by the reaction of the dye and glycans to produce stable fluorescently labeled glycan

2-AB Labeling Kit

2-AB (2-aminobenzamide) is one of the most widely used fluorescent labels for glycosylation analysis

Photo Guide of the Procedure

View product documentation as PDF

View Certificate of Analysis as PDF

Part Number: LT-KAB-A2

2-AB Kit with Non-Toxic Reductant

2-AB (2-aminobenzamide) labeling using a non-toxic reductant, 2-picoline borane.

Photo Guide of the Procedure

View product documentation as PDF

View Certificate of Analysis as PDF

Part Number: LT-KAB-VP24

2-AA Labeling Kit

2-AA (2-aminobenzoic acid) is considered by many to be a superior replacement for 2-AB (2-aminobenzamide) in most types of complex glycan analysis. 2-AA is reported to have a higher fluorescence and gives higher labelling efficiencies than 2-AB.

View protocol picture guide as a PDF

View product documentation as PDF

View Certificate of Analysis as PDF

Part Number: LT-KAA-A2

2-AA Kit with Non-Toxic Reductant

2-AA (2-aminobenzoic acid) labeling using a non-toxic reductant, 2-picoline borane

Photo Guide of the Procedure

View product documentation as PDF

View Certificate of Analysis as PDF

Part Number: LT-KAA-VP24

LudgerTag Monosaccharide Kit

This kit contains reagents for the release and fluorescent labeling of monosaccharides found on glycoprotein biopharmaceuticals and standards. View product documentation as PDF View Certificate of Analysis as PDF Part Number: LT-MONO-96
$1,274.00

DMB Sialic Acid Labeling Kit

The LudgerTag DMB Labeling Kit has been developed for the qualitative analysis of sialic acids. The kit includes all the reagents to release sialic acids from your glycoprotein and label these with DMB. View product documentation as PDF View Certificate of Analysis as PDF Part Number: LT-KDMB-A1

Procainamide Labeling Kit

Procainamide labelling permits glycan identification by either mass spectrometry or UHPLC. Because of its improved ionisation efficiency compared to 2AB labelling, it can permit identification of minor glycans (1% relative peak area) by ESI-MS. This LudgerTag kit, along with a post-labelling clean up plate for the samples (catalog# LC-PROC-96), has been validated in house at Ludger. Typical CVs for triplicate analyses were 5%

Photo Guide of the Procedure

View product documentation as PDF

View Certificate of Analysis as PDF

Part Number: LT-KPROC-VP24

V-Tag Glycopeptide Labeling Kit

The LudgerTag V-tag labeling kit is designed for the fluorophore labeling of enzymatically digested peptides and glycopeptides followed by enrichment of the labeled glycopeptides.

Photo Guide of the Procedure

View product documentation as PDF

View Certificate of Analysis as PDF

Part Number: LT-VTAG-24

$1,273.00
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